Composite

Part:BBa_K1520006

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-22)

Prlux-rbs-cI-Ter

Prlux-rbs-cI-Ter. It produces cI when there is enough luxR and AHL.

K152006.jpg

From left to right, Marker（DL2000）, Prlux, Prlux-rbs-cI, Prlux-rbs-cI-T. The substances of electrophoresis are made from PCR of plasmids with VF2 and VR as their primers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
Parameters
controlluxR, HSL
directionForward
functionRep
ligandsRecA
negative_regulators
o_h
o_l
positive_regulators1
proteinCI lambda
swissproP03034
tagLVA